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High Content Imaging

High Content Imaging

The Facility has a Molecular Devices ImageXpress HT.ai which is a confocal and wide-field imaging system specifically designed to image cells cultured in standard format multi-well plates (although it can also image cells on slides).  The HT.ai is equipped with a robotic plate loader which significantly increases the throughput capability of the system.  Potential applications for this system include live/dead analysis, cell cycle analysis, apoptosis, cytoplasmic/ nucleus translocation assays, DNA damage, tube formation (angiogenesis), neurite outgrowth, micronucleus assays, morphometric analysis (tubulin) and cell migration.

Our System:

Molecular Devices HT.ai

Molecular Devices HT.ai

Wide-field and confocal automated imaging system

405, 445, 475, 520, 555, 640, 730 nm lasers

4x, 10x, 20x water immersion, 40x objectives

Compatible with all standard tissue culture plates formats (384-well, 96-well, 24-well etc.)

Robotic loader with 40 plate capacity